Effects of Cigarette Smoke on TSPO-related Mitochondrial Processes

• Main Authors: Nidal Zeineh, Rafael Nagler, Martin Gabay, Abraham Weizman, Moshe Gavish
• Format: Article
• Language: English
• Published: MDPI AG 2019-07-01
• Series: Cells
• Subjects: TSPO; cAMP; cigarette smoke; mitochondrial membrane potential; ROS; apoptosis; necrosis; cell death
• Online Access: https://www.mdpi.com/2073-4409/8/7/694

The 18 kDa translocator protein (TSPO) is an initiator of the mitochondrial apoptosis cascade. Cigarette smoke (CS) exposure provokes alterations in TSPO expression as well as upregulation of its related functions such as mitochondrial membrane potential (&#916;&#968;_M) and reactive oxygen species generation, which are associated with cell death. In the current study, H1299 lung cancer cell line exposed to CS for various time periods (30 mins, 60 mins and 120 mins) and TSPO expression and cell death processes were studied. CS exposure for 30 mins resulted in a non-significant increase in TSPO expression by 24% (<i>p</i> &gt; 0.05 vs. control). CS exposure for 60 mins and 120 mins resulted in a significant increase by 43% (<i>p</i> &lt; 0.05 vs. control) and by 47% (<i>p</i> &lt; 0.01 vs. control), respectively. Furthermore, TSPO-related mitochondrial functions were upregulated at the 120 mins time point following CS exposure. TSPO expression is upregulated by CS, suggesting that TSPO plays a role in cell death processes induced by CS exposure. Alterations in TSPO-related cell death processes suggest that TSPO may be involved in the tissue damage caused by CS.