Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

Abstract Background Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only rudimentary. We adapt molecular genetics meth...

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Bibliographic Details
Main Authors: Oliver Blechert, Huan Mei, Xiaohui Zang, Hailin Zheng, Guanzhao Liang, Weida Liu
Format: Article
Language:English
Published: BMC 2020-01-01
Series:BMC Biotechnology
Subjects:
Trichophyton rubrum
Cas9 ribonucleoprotein complex
Gene knock-out
Uracil
Tryptophan
Online Access:https://doi.org/10.1186/s12896-020-0601-z

Internet

https://doi.org/10.1186/s12896-020-0601-z

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