Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

Abstract This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that co...

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Bibliographic Details
Main Authors: Aleta R. Steevens, Matthew W. Griesbach, Yun You, James R. Dutton, Walter C. Low, Peter A. Santi
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Stem Cell Research & Therapy
Subjects:
Inner ear
Spiral ganglion neurons
Neurogenin1
Blastocyst complementation
Stem cells
Regenerative medicine
Online Access:https://doi.org/10.1186/s13287-021-02184-1
Description
Summary:Abstract This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.
ISSN:1757-6512

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