Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

Abstract This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that co...

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Main Authors:	Aleta R. Steevens, Matthew W. Griesbach, Yun You, James R. Dutton, Walter C. Low, Peter A. Santi
Format:	Article
Language:	English
Published:	BMC 2021-02-01
Series:	Stem Cell Research & Therapy
Subjects:	Inner ear Spiral ganglion neurons Neurogenin1 Blastocyst complementation Stem cells Regenerative medicine
Online Access:	https://doi.org/10.1186/s13287-021-02184-1

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spelling	doaj-52f702c5d50c40be8ec82d0efb64cfa62021-02-14T12:08:37ZengBMCStem Cell Research & Therapy1757-65122021-02-0112111210.1186/s13287-021-02184-1Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouseAleta R. Steevens0Matthew W. Griesbach1Yun You2James R. Dutton3Walter C. Low4Peter A. Santi5Department of Ophthalmology, University of MinnesotaDepartment of Otolaryngology, University of MinnesotaMouse Genetics Laboratory, University of MinnesotaStem Cell Institute, University of MinnesotaDepartment of Neurosurgery, University of MinnesotaStem Cell Institute, University of MinnesotaAbstract This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.https://doi.org/10.1186/s13287-021-02184-1Inner earSpiral ganglion neuronsNeurogenin1Blastocyst complementationStem cellsRegenerative medicine
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Aleta R. Steevens Matthew W. Griesbach Yun You James R. Dutton Walter C. Low Peter A. Santi
spellingShingle	Aleta R. Steevens Matthew W. Griesbach Yun You James R. Dutton Walter C. Low Peter A. Santi Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse Stem Cell Research & Therapy Inner ear Spiral ganglion neurons Neurogenin1 Blastocyst complementation Stem cells Regenerative medicine
author_facet	Aleta R. Steevens Matthew W. Griesbach Yun You James R. Dutton Walter C. Low Peter A. Santi
author_sort	Aleta R. Steevens
title	Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse
title_short	Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse
title_full	Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse
title_fullStr	Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse
title_full_unstemmed	Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse
title_sort	generation of inner ear sensory neurons using blastocyst complementation in a neurog1 +/−−deficient mouse
publisher	BMC
series	Stem Cell Research & Therapy
issn	1757-6512
publishDate	2021-02-01
description	Abstract This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.
topic	Inner ear Spiral ganglion neurons Neurogenin1 Blastocyst complementation Stem cells Regenerative medicine
url	https://doi.org/10.1186/s13287-021-02184-1
work_keys_str_mv	AT aletarsteevens generationofinnerearsensoryneuronsusingblastocystcomplementationinaneurog1deficientmouse AT matthewwgriesbach generationofinnerearsensoryneuronsusingblastocystcomplementationinaneurog1deficientmouse AT yunyou generationofinnerearsensoryneuronsusingblastocystcomplementationinaneurog1deficientmouse AT jamesrdutton generationofinnerearsensoryneuronsusingblastocystcomplementationinaneurog1deficientmouse AT walterclow generationofinnerearsensoryneuronsusingblastocystcomplementationinaneurog1deficientmouse AT peterasanti generationofinnerearsensoryneuronsusingblastocystcomplementationinaneurog1deficientmouse
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Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

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