Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency.

Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency.

CRISPR/Cas9 allows the generation of knockout cell lines and null zygotes by inducing site-specific double-stranded breaks. In most cases the DSB is repaired by non-homologous end joining, resulting in small nucleotide insertions or deletions that can be used to construct knockout alleles. However,...

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Bibliographic Details
Main Authors: Ignacio García-Tuñón, Verónica Alonso-Pérez, Elena Vuelta, Sandra Pérez-Ramos, María Herrero, Lucía Méndez, Jesús María Hernández-Sánchez, Marta Martín-Izquierdo, Raquel Saldaña, Julián Sevilla, Fermín Sánchez-Guijo, Jesús María Hernández-Rivas, Manuel Sánchez-Martín
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0216674

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https://doi.org/10.1371/journal.pone.0216674

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