Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells

Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells

Abstract Background Precise genome editing is essential for both basic and translational research. The recently developed CRISPR/Cas9 system can specifically cleave a designated site of target gene to create a DNA double-strand break, which triggers cellular DNA repair mechanism of either inaccurate...

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Bibliographic Details
Main Authors: Zheng Hu, Zhaoying Shi, Xiaogang Guo, Baishan Jiang, Guo Wang, Dixian Luo, Yonglong Chen, Yuan-Shan Zhu
Format: Article
Language:English
Published: BMC 2018-02-01
Series:Cell & Bioscience
Subjects:
CRISPR/Cas9
Homology-directed repair
DNA ligase IV inhibitor
Single-stranded oligodeoxynucleotides (ssODN)
Non-homologous DNA end joining
Online Access:http://link.springer.com/article/10.1186/s13578-018-0200-z

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http://link.springer.com/article/10.1186/s13578-018-0200-z

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