Reversible Cryopreservation of Living Cells Using an Electron Microscopy Cryo-Fixation Method.

Rapid cooling of aqueous solutions is a useful approach for two important biological applications: (I) cryopreservation of cells and tissues for long-term storage, and (II) cryofixation for ultrastructural investigations by electron and cryo-electron microscopy. Usually, both approaches are very dif...

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Bibliographic Details
Main Authors: Jan Huebinger, Hong-Mei Han, Markus Grabenbauer
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5053471?pdf=render