The construction of a secretary eukaryotic expression plasmid of chondroitinase ABC Ⅰ

Objective To construct a secretary eukaryotic expression plasmid for chondroitinase ABCⅠ, and observe the expression of the target protein in a glioma cell line TJ905. Methods The DNA fragments encoding the signal peptide of basilar membrane 40 (BM40) as well as the mature fragment of chondroitinase...

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Bibliographic Details
Main Authors: Hui⁃ling GAO, Qian WANG, Shi⁃zhu YU, Xiu⁃ju CHEN, Cui⁃yun SUN, Tong⁃ling AN
Format: Article
Language:English
Published: Tianjin Huanhu Hospital 2010-08-01
Series:Chinese Journal of Contemporary Neurology and Neurosurgery
Subjects:
Online Access:http://www.cjcnn.org/index.php/cjcnn/article/view/488