The construction of a secretary eukaryotic expression plasmid of chondroitinase ABC Ⅰ
Objective To construct a secretary eukaryotic expression plasmid for chondroitinase ABCⅠ, and observe the expression of the target protein in a glioma cell line TJ905. Methods The DNA fragments encoding the signal peptide of basilar membrane 40 (BM40) as well as the mature fragment of chondroitinase...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Tianjin Huanhu Hospital
2010-08-01
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Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
Subjects: | |
Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/488 |