Development of a Method for Embedded-Culture of Pig Pancreatic Islet-Like Cell Clusters in Agarose Containing Maltose-Carrying Polystyrene (HEVM) and Nicotinamide
A method for embedded-culture of islet-like cell clusters (ICCs) from neonatal pig pancreas is described. The procedure is based on a sequential treatment of the pancreas with a proteolytic enzyme and ethylenediaminetetraacetate (EDTA). During the exposure to EDTA-Dispase, small pieces of pancreas w...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
1994-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979400300112 |