UV-Denaturation Assay to Assess Protein Photostability and Ligand-Binding Interactions Using the High Photon Flux of Diamond B23 Beamline for SRCD

UV-Denaturation Assay to Assess Protein Photostability and Ligand-Binding Interactions Using the High Photon Flux of Diamond B23 Beamline for SRCD

Light irradiation with high photon flux in the vacuum and far-UV region is known to denature the conformation of biopolymers. Measures are in place at Diamond Light Source B23 beamline for Synchrotron Radiation Circular Dichroism (SRCD) to control and make this effect negligible. However, UV denatur...

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Bibliographic Details
Main Authors: Rohanah Hussain, Edoardo Longo, Giuliano Siligardi
Format: Article
Language:English
Published: MDPI AG 2018-07-01
Series:Molecules
Subjects:
circular dichroism
ligand binding
high photon flux
protein stability
synchrotron radiation
SRCD
vacuum UV
Online Access:http://www.mdpi.com/1420-3049/23/8/1906

Internet

http://www.mdpi.com/1420-3049/23/8/1906

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