Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy

Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy

Abstract Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary. We propose to use polarization-dependent fluorescence correlation spectroscopy (Pol-FCS) for evalu...

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Bibliographic Details
Main Authors: Johtaro Yamamoto, Akito Matsui, Fusako Gan, Makoto Oura, Riku Ando, Takahiro Matsuda, Jian Ping Gong, Masataka Kinjo
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-89987-7

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https://doi.org/10.1038/s41598-021-89987-7

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