An optimized dissociation protocol for FACS-based isolation of rare cell types from Caenorhabditis elegans L1 larvae

Single-cell isolation and transcriptomic analysis of a specific cell type or tissue offers the possibility of studying cell function and heterogeneity in time-dependent processes with remarkable resolution. The reduced tissue complexity and highly stereotyped development of Caenorhabditis elegans, c...

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Bibliographic Details
Main Authors: Euclides E. Fernandes Póvoa, Annabel L.P. Ebbing, Marco C. Betist, Christa van der Veen, Hendrik C. Korswagen
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016120301412