Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties

Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties

Fcɤ receptors (FcɤR) mediate key functions in immunological responses. For instance, FcɤRIIIa is involved in antibody-dependent cell-mediated cytotoxicity (ADCC). FcɤRIIIa interacts with the fragment crystallizable (Fc) of immunoglobulin G (IgG). This interaction is known to be highly dependent on I...

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Bibliographic Details
Main Authors: Steffen Lippold, Simone Nicolardi, Manfred Wuhrer, David Falck
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Chemistry
Subjects:
affinity chromatography
mass spectrometry
middle-up protein analysis
cetuximab
Fc glycosylation
Fab glycosylation
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2019.00698/full

Internet

https://www.frontiersin.org/article/10.3389/fchem.2019.00698/full

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