FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons

FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons

The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion...

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Bibliographic Details
Main Authors: Carles eBosch, Albert eMartínez, Núria eMasachs, Cátia M Teixeira, Isabel eFernaud, Fausto eUlloa, Esther ePérez, Carlos eLois, Joan X. Comella, Javier eDeFelipe, Angel eMerchan-Perez, Eduardo eSoriano
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-05-01
Series:Frontiers in Neuroanatomy
Subjects:
Dendritic Spines
Synapses
adult neurogenesis
3D-reconstruction
Electron microscopy
FIB/SEM
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00060/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00060/full

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