Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2003-03-01
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| Series: | Memórias do Instituto Oswaldo Cruz. |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017 |