Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor

In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose an...

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Bibliographic Details
Main Author: John Graham
Format: Article
Language:English
Published: Hindawi Limited 2002-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2002.838