Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose an...
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2002-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1100/tsw.2002.838 |