Combining laser-assisted microdissection (LAM) and RNA-seq allows to perform a comprehensive transcriptomic analysis of epidermal cells of Arabidopsis embryo

Combining laser-assisted microdissection (LAM) and RNA-seq allows to perform a comprehensive transcriptomic analysis of epidermal cells of Arabidopsis embryo

Abstract Background Genome-wide characterization of tissue- or cell-specific gene expression is a recurrent bottleneck in biology. We have developed a sensitive approach based on ultra-low RNA sequencing coupled to laser assisted microdissection for analyzing different tissues of the small Arabidops...

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Bibliographic Details
Main Authors: Kaori Sakai, Ludivine Taconnat, Nero Borrega, Jennifer Yansouni, Véronique Brunaud, Christine Paysant-Le Roux, Etienne Delannoy, Marie-Laure Martin Magniette, Loïc Lepiniec, Jean Denis Faure, Sandrine Balzergue, Bertrand Dubreucq
Format: Article
Language:English
Published: BMC 2018-02-01
Series:Plant Methods
Subjects:
Laser-assisted microdissection
Ultra-low-RNA-seq
Epidermis
Embryo cells
Arabidopsis
Online Access:http://link.springer.com/article/10.1186/s13007-018-0275-x

Internet

http://link.springer.com/article/10.1186/s13007-018-0275-x

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