Development of an Improved Peroxidase-Based High-Throughput Screening for the Optimization of D-Glycerate Dehydratase Activity

Development of an Improved Peroxidase-Based High-Throughput Screening for the Optimization of D-Glycerate Dehydratase Activity

Successful directed evolution examples span a broad range of improved enzyme properties. Nevertheless, the most challenging step for each single directed evolution approach is an efficient identification of improved variants from a large genetic library. Thus, the development and choice of a proper...

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Bibliographic Details
Main Authors: Benjamin Begander, Anna Huber, Manuel Döring, Josef Sperl, Volker Sieber
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
enzyme engineering
high-throughput screening
biocatalysis
dehydratases
Online Access:https://www.mdpi.com/1422-0067/21/1/335

Internet

https://www.mdpi.com/1422-0067/21/1/335

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