System-wide identification and prioritization of enzyme substrates by thermal analysis

System-wide identification and prioritization of enzyme substrates by thermal analysis

The global identification of enzyme substrates is still challenging. Here, the authors develop a method based on proteome-wide thermal shift assays to discover enzyme substrates directly from cell lysates, identifying known and novel oxidoreductase, kinase and poly-(ADP-ribose) polymerase substrates...

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Bibliographic Details
Main Authors: Amir Ata Saei, Christian M. Beusch, Pierre Sabatier, Juan Astorga Wells, Hassan Gharibi, Zhaowei Meng, Alexey Chernobrovkin, Sergey Rodin, Katja Näreoja, Ann-Gerd Thorsell, Tobias Karlberg, Qing Cheng, Susanna L. Lundström, Massimiliano Gaetani, Ákos Végvári, Elias S. J. Arnér, Herwig Schüler, Roman A. Zubarev
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21540-6

Internet

https://doi.org/10.1038/s41467-021-21540-6

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