Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice
Age-associated loss of retinal ganglion cells (RGCs) causes visual deficits, but there is not yet any therapeutic agent to prevent the loss of these cells. Herein, we report that apelin, an endogenous peptide ligand of APJ receptor, is protective against the age-related loss of RGCs in mice. The mRN...
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doaj-56ae3a1745c8470bbe8bf4e95daaa9792020-11-25T03:15:39ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652020-03-011210.3389/fnagi.2020.00058485961Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in MiceYuki Ishimaru0Akihide Sumino1Akihide Sumino2Fumiya Shibagaki3Akiko Yamamuro4Yasuhiro Yoshioka5Sadaaki Maeda6Laboratory of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, JapanLaboratory of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, JapanLaboratory of Food Chemistry, Yokohama University of Pharmacy, Yokohama, JapanLaboratory of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, JapanLaboratory of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, JapanLaboratory of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, JapanLaboratory of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, JapanAge-associated loss of retinal ganglion cells (RGCs) causes visual deficits, but there is not yet any therapeutic agent to prevent the loss of these cells. Herein, we report that apelin, an endogenous peptide ligand of APJ receptor, is protective against the age-related loss of RGCs in mice. The mRNA expression of apelin was reduced in the retina of old mice compared with that in young mice, whereas retinal APJ expression increased with age. Immunofluorescence staining showed that APJ was present in RGCs and their surrounding cells expressed apelin. In addition, both functional and histological analyses demonstrated that apelin deficiency accelerated the loss of RGCs associated with age in mice. These results suggest that endogenous apelin plays a protective role against the degeneration of RGCs and that the apelinergic axis may be a new target for preventing age-related visual impairment.https://www.frontiersin.org/article/10.3389/fnagi.2020.00058/fullretinal ganglion cell (RGC)apelinagingknock-outcell lossapelin receptor (APJ) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuki Ishimaru Akihide Sumino Akihide Sumino Fumiya Shibagaki Akiko Yamamuro Yasuhiro Yoshioka Sadaaki Maeda |
spellingShingle |
Yuki Ishimaru Akihide Sumino Akihide Sumino Fumiya Shibagaki Akiko Yamamuro Yasuhiro Yoshioka Sadaaki Maeda Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice Frontiers in Aging Neuroscience retinal ganglion cell (RGC) apelin aging knock-out cell loss apelin receptor (APJ) |
author_facet |
Yuki Ishimaru Akihide Sumino Akihide Sumino Fumiya Shibagaki Akiko Yamamuro Yasuhiro Yoshioka Sadaaki Maeda |
author_sort |
Yuki Ishimaru |
title |
Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice |
title_short |
Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice |
title_full |
Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice |
title_fullStr |
Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice |
title_full_unstemmed |
Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice |
title_sort |
endogenous apelin is protective against age-associated loss of retinal ganglion cells in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Aging Neuroscience |
issn |
1663-4365 |
publishDate |
2020-03-01 |
description |
Age-associated loss of retinal ganglion cells (RGCs) causes visual deficits, but there is not yet any therapeutic agent to prevent the loss of these cells. Herein, we report that apelin, an endogenous peptide ligand of APJ receptor, is protective against the age-related loss of RGCs in mice. The mRNA expression of apelin was reduced in the retina of old mice compared with that in young mice, whereas retinal APJ expression increased with age. Immunofluorescence staining showed that APJ was present in RGCs and their surrounding cells expressed apelin. In addition, both functional and histological analyses demonstrated that apelin deficiency accelerated the loss of RGCs associated with age in mice. These results suggest that endogenous apelin plays a protective role against the degeneration of RGCs and that the apelinergic axis may be a new target for preventing age-related visual impairment. |
topic |
retinal ganglion cell (RGC) apelin aging knock-out cell loss apelin receptor (APJ) |
url |
https://www.frontiersin.org/article/10.3389/fnagi.2020.00058/full |
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