An improved method for extraction of microbial DNA from alkaline-saline soil

An improved method for extraction of microbial DNA from alkaline-saline soil

A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (Mo Bio™ Laboratories, Inc.) was used as a ref...

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Bibliographic Details
Main Authors: Valentín Pérez-Hernández, Mario Hernández-Guzmán, César Valenzuela-Encinas, Rocío Alcántara-Hernández, Isabel Estrada-Alvarado, Luc Dendooven, Rodolfo Marsch, Federico Gutiérrez-Miceli, Víctor M. Ruíz-Valdiviezo, Joaquín A. Montes-Molina
Format: Article
Language:English
Published: Sociedad Mexicana de la Ciencia del Suelo A. C. 2021-06-01
Series:Terra Latinoamericana
Subjects:
dna extraction
dna sequencing
pcr amplification
saline-alkaline soil
Online Access:https://www.terralatinoamericana.org.mx/index.php/terra/article/view/887

Internet

https://www.terralatinoamericana.org.mx/index.php/terra/article/view/887

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