Reporter Assay for Endo/Lysosomal Escape of Toxin-Based Therapeutics

Reporter Assay for Endo/Lysosomal Escape of Toxin-Based Therapeutics

Protein-based therapeutics with cytosolic targets are capable of exhibiting their therapeutic effect once they have escaped from the endosomes or lysosomes. In this study, the reporters—horseradish peroxidase (HRP), Alexa Fluor 488 (Alexa) and ricin A-chain (RTA)—were investigated for their capacity...

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Bibliographic Details
Main Authors: Roger Gilabert-Oriol, Mayank Thakur, Benedicta von Mallinckrodt, Cheenu Bhargava, Burkhard Wiesner, Jenny Eichhorst, Matthias F. Melzig, Hendrik Fuchs, Alexander Weng
Format: Article
Language:English
Published: MDPI AG 2014-05-01
Series:Toxins
Subjects:
endosomal escape
reporter assay
protein therapeutics
ribosome inactivating proteins
horseradish peroxidase
Alexa Fluor 488
ricin A-chain
saporin
triterpenoidal saponins
Online Access:http://www.mdpi.com/2072-6651/6/5/1644

Internet

http://www.mdpi.com/2072-6651/6/5/1644

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