The stochastic nature of errors in next-generation sequencing of circulating cell-free DNA.

The stochastic nature of errors in next-generation sequencing of circulating cell-free DNA.

Challenges with distinguishing circulating tumor DNA (ctDNA) from next-generation sequencing (NGS) artifacts limits variant searches to established solid tumor mutations. Here we show early and random PCR errors are a principal source of NGS noise that persist despite duplex molecular barcoding, rem...

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Bibliographic Details
Main Authors: David A Nix, Sabine Hellwig, Christopher Conley, Alun Thomas, Carrie L Fuertes, Cindy L Hamil, Preetida J Bhetariya, Ignacio Garrido-Laguna, Gabor T Marth, Mary P Bronner, Hunter R Underhill
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0229063

Internet

https://doi.org/10.1371/journal.pone.0229063

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