Genetic Dissection of a Prevalent Plasmid-Encoded Conjugation System in Lactococcus lactis

Genetic Dissection of a Prevalent Plasmid-Encoded Conjugation System in Lactococcus lactis

Plasmid pNP40, which was first identified nearly 40 years ago in Lactococcus lactis subsp. lactis biovar diacetylactis DRC3, encodes functions such as heavy metal-, bacteriophage-, and nisin-resistance, as well as plasmid transfer ability by conjugation. Here, we report an optimized conjugation prot...

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Bibliographic Details
Main Authors: Guillermo Ortiz Charneco, Philip Kelleher, Andrius Buivydas, Hugo Streekstra, Emiel Ver Loren van Themaat, Paul P. de Waal, Jennifer Mahony, Douwe van Sinderen
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Microbiology
Subjects:
lactococci
starter culture
dairy fermentation
conjugative plasmid
recombineering
mutagenesis
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.680920/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.680920/full

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