Is “cooling then freezing” a humane way to kill amphibians and reptiles?

What is the most humane way to kill amphibians and small reptiles that are used in research? Historically, such animals were often killed by cooling followed by freezing, but this method was outlawed by ethics committees because of concerns that ice-crystals may form in peripheral tissues while the...

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Bibliographic Details
Main Authors: Richard Shine, Joshua Amiel, Adam J. Munn, Mathew Stewart, Alexei L. Vyssotski, John A. Lesku
Format: Article
Language:English
Published: The Company of Biologists 2015-07-01
Series:Biology Open
Subjects:
Online Access:http://bio.biologists.org/content/4/7/760