Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification

There is a need to further improve the automation of cryo-EM sample preparation to make it more easily accessible for non-specialists, reduce sample waste and increase reproducibility. Here, the authors present VitroJet, a single device, where sub-nl volumes of samples are deposited by pin printing...

Full description

Bibliographic Details
Main Authors: Raimond B. G. Ravelli, Frank J. T. Nijpels, Rene J. M. Henderikx, Giulia Weissenberger, Sanne Thewessem, Abril Gijsbers, Bart W. A. M. M. Beulen, Carmen López-Iglesias, Peter J. Peters
Format: Article
Language:English
Published: Nature Publishing Group 2020-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-16392-5