The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies
Osteoarthritis (OA), defined as a long-term progressive joint disease, is characterized by cartilage impairment and erosion. In recent decades, magnolol, as a type of lignin extracted from Magnolia officinalis, has been proved to play a potent anti-inflammatory role in various diseases. The current...
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Frontiers Media S.A.
2019-04-01
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Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00393/full |
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language |
English |
format |
Article |
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DOAJ |
author |
Zhi-Chao Hu Zhi-Chao Hu Zhi-Chao Hu Zu-Cheng Luo Zu-Cheng Luo Zu-Cheng Luo Bing-Jie Jiang Bing-Jie Jiang Bing-Jie Jiang Xin Fu Xin Fu Xin Fu Jiang-Wei Xuan Jiang-Wei Xuan Jiang-Wei Xuan Xiao-Bin Li Xiao-Bin Li Xiao-Bin Li Yu-Jie Bian Yu-Jie Bian Yu-Jie Bian Wen-Fei Ni Wen-Fei Ni Wen-Fei Ni Ji-Xin Xue Ji-Xin Xue Ji-Xin Xue |
spellingShingle |
Zhi-Chao Hu Zhi-Chao Hu Zhi-Chao Hu Zu-Cheng Luo Zu-Cheng Luo Zu-Cheng Luo Bing-Jie Jiang Bing-Jie Jiang Bing-Jie Jiang Xin Fu Xin Fu Xin Fu Jiang-Wei Xuan Jiang-Wei Xuan Jiang-Wei Xuan Xiao-Bin Li Xiao-Bin Li Xiao-Bin Li Yu-Jie Bian Yu-Jie Bian Yu-Jie Bian Wen-Fei Ni Wen-Fei Ni Wen-Fei Ni Ji-Xin Xue Ji-Xin Xue Ji-Xin Xue The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies Frontiers in Pharmacology magnolol PI3K/Akt/NF-κB osteoarthritis interleukin-1 beta inflammation |
author_facet |
Zhi-Chao Hu Zhi-Chao Hu Zhi-Chao Hu Zu-Cheng Luo Zu-Cheng Luo Zu-Cheng Luo Bing-Jie Jiang Bing-Jie Jiang Bing-Jie Jiang Xin Fu Xin Fu Xin Fu Jiang-Wei Xuan Jiang-Wei Xuan Jiang-Wei Xuan Xiao-Bin Li Xiao-Bin Li Xiao-Bin Li Yu-Jie Bian Yu-Jie Bian Yu-Jie Bian Wen-Fei Ni Wen-Fei Ni Wen-Fei Ni Ji-Xin Xue Ji-Xin Xue Ji-Xin Xue |
author_sort |
Zhi-Chao Hu |
title |
The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies |
title_short |
The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies |
title_full |
The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies |
title_fullStr |
The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies |
title_full_unstemmed |
The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo Studies |
title_sort |
protective effect of magnolol in osteoarthritis: in vitro and in vivo studies |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2019-04-01 |
description |
Osteoarthritis (OA), defined as a long-term progressive joint disease, is characterized by cartilage impairment and erosion. In recent decades, magnolol, as a type of lignin extracted from Magnolia officinalis, has been proved to play a potent anti-inflammatory role in various diseases. The current research sought to examine the latent mechanism of magnolol and its protective role in alleviating the progress of OA in vivo as well as in vitro experimentations. In vitro, the over-production of Nitric oxide (NO), prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), and interleukin-6 (IL-6), induced by interleukin-1 beta (IL-1β), were all inhibited notably by magnolol in a concentration-dependent manner. Moreover, magnolol could also downregulate the expression of metalloproteinase 13 (MMP13) and thrombospondin motifs 5 (ADAMTS5). All these changes ultimately led to the deterioration of the extracellular matrix (ECM) induced by IL-1β. Mechanistically, magnolol suppressed the activation of PI3K/Akt/NF-κB pathway. Furthermore, a powerful binding capacity between magnolol and PI3K was also revealed in our molecular docking research. In addition, magnolol-induced protective effects in OA development were also detected in a mouse model. In summary, this research suggested that magnolol possessed a new therapeutic potential for the development of OA. |
topic |
magnolol PI3K/Akt/NF-κB osteoarthritis interleukin-1 beta inflammation |
url |
https://www.frontiersin.org/article/10.3389/fphar.2019.00393/full |
work_keys_str_mv |
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doaj-577463cb21b94fcca7c42be0fa71ba3a2020-11-25T00:19:00ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-04-011010.3389/fphar.2019.00393453663The Protective Effect of Magnolol in Osteoarthritis: In vitro and in vivo StudiesZhi-Chao Hu0Zhi-Chao Hu1Zhi-Chao Hu2Zu-Cheng Luo3Zu-Cheng Luo4Zu-Cheng Luo5Bing-Jie Jiang6Bing-Jie Jiang7Bing-Jie Jiang8Xin Fu9Xin Fu10Xin Fu11Jiang-Wei Xuan12Jiang-Wei Xuan13Jiang-Wei Xuan14Xiao-Bin Li15Xiao-Bin Li16Xiao-Bin Li17Yu-Jie Bian18Yu-Jie Bian19Yu-Jie Bian20Wen-Fei Ni21Wen-Fei Ni22Wen-Fei Ni23Ji-Xin Xue24Ji-Xin Xue25Ji-Xin Xue26Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second School of Medicine, Wenzhou Medical University, Wenzhou, ChinaBone Research Institute, The Key Orthopaedic Laboratory of Zhejiang Province, Wenzhou, ChinaOsteoarthritis (OA), defined as a long-term progressive joint disease, is characterized by cartilage impairment and erosion. In recent decades, magnolol, as a type of lignin extracted from Magnolia officinalis, has been proved to play a potent anti-inflammatory role in various diseases. The current research sought to examine the latent mechanism of magnolol and its protective role in alleviating the progress of OA in vivo as well as in vitro experimentations. In vitro, the over-production of Nitric oxide (NO), prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α), and interleukin-6 (IL-6), induced by interleukin-1 beta (IL-1β), were all inhibited notably by magnolol in a concentration-dependent manner. Moreover, magnolol could also downregulate the expression of metalloproteinase 13 (MMP13) and thrombospondin motifs 5 (ADAMTS5). All these changes ultimately led to the deterioration of the extracellular matrix (ECM) induced by IL-1β. Mechanistically, magnolol suppressed the activation of PI3K/Akt/NF-κB pathway. Furthermore, a powerful binding capacity between magnolol and PI3K was also revealed in our molecular docking research. In addition, magnolol-induced protective effects in OA development were also detected in a mouse model. In summary, this research suggested that magnolol possessed a new therapeutic potential for the development of OA.https://www.frontiersin.org/article/10.3389/fphar.2019.00393/fullmagnololPI3K/Akt/NF-κBosteoarthritisinterleukin-1 betainflammation |