Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System
This study reports the use of the BacMam system to deliver and express self-assembling IL-15 and IL-15Rα genes to murine B16F10 melanoma and CT26 colon cancer cells. BacMam-based IL-15 and IL-15Rα were well-expressed and assembled to form the biologically functional IL-15:IL-15Rα complex. Immunizati...
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doaj-577dd3a6efcc4e65ac224fcbcf8023332021-08-26T13:35:35ZengMDPI AGCancers2072-66942021-08-01134039403910.3390/cancers13164039Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus SystemVan Anh Do-Thi0Hayyoung Lee1Hye Jin Jeong2Jie-Oh Lee3Young Sang Kim4Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, KoreaInstitute of Biotechnology, Chungnam National University, Daejeon 34134, KoreaDepartment of Life Sciences, Pohang University of Science and Technology, Pohang 37673, KoreaDepartment of Life Sciences, Pohang University of Science and Technology, Pohang 37673, KoreaDepartment of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 34134, KoreaThis study reports the use of the BacMam system to deliver and express self-assembling IL-15 and IL-15Rα genes to murine B16F10 melanoma and CT26 colon cancer cells. BacMam-based IL-15 and IL-15Rα were well-expressed and assembled to form the biologically functional IL-15:IL-15Rα complex. Immunization with this IL-15:IL-15Rα cancer vaccine delayed tumor growth in mice by inducing effector memory CD4<sup>+</sup> and CD8<sup>+</sup> cells and effector NK cells which are tumor-infiltrating. It caused strong antitumor immune responses of CD8<sup>+</sup> effector cells in a tumor-antigen specific manner both in vitro and in vivo and significantly attenuated Treg cells which a control virus-infected cancer vaccine could induce. Post-treatment with this cancer vaccine after a live cancer cell injection also prominently delayed the growth of the tumor. Collectively, we demonstrate a vaccine platform consisting of BacMam virus-infected B16F10 or CT26 cancer cells that secrete IL-15:IL-15Rα. This study is the first demonstration of a functionally competent soluble IL-15:IL-15Rα complex-related cancer vaccine using a baculovirus system and advocates that the BacMam system can be used as a secure and rapid method of producing a protective and therapeutic cancer vaccine.https://www.mdpi.com/2072-6694/13/16/4039interleukin 15interleukin 15RαB16F10 melanomaCT-26cytokineanticancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Van Anh Do-Thi Hayyoung Lee Hye Jin Jeong Jie-Oh Lee Young Sang Kim |
spellingShingle |
Van Anh Do-Thi Hayyoung Lee Hye Jin Jeong Jie-Oh Lee Young Sang Kim Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System Cancers interleukin 15 interleukin 15Rα B16F10 melanoma CT-26 cytokine anticancer |
author_facet |
Van Anh Do-Thi Hayyoung Lee Hye Jin Jeong Jie-Oh Lee Young Sang Kim |
author_sort |
Van Anh Do-Thi |
title |
Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System |
title_short |
Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System |
title_full |
Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System |
title_fullStr |
Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System |
title_full_unstemmed |
Protective and Therapeutic Effects of an IL-15:IL-15Rα-Secreting Cell-Based Cancer Vaccine Using a Baculovirus System |
title_sort |
protective and therapeutic effects of an il-15:il-15rα-secreting cell-based cancer vaccine using a baculovirus system |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2021-08-01 |
description |
This study reports the use of the BacMam system to deliver and express self-assembling IL-15 and IL-15Rα genes to murine B16F10 melanoma and CT26 colon cancer cells. BacMam-based IL-15 and IL-15Rα were well-expressed and assembled to form the biologically functional IL-15:IL-15Rα complex. Immunization with this IL-15:IL-15Rα cancer vaccine delayed tumor growth in mice by inducing effector memory CD4<sup>+</sup> and CD8<sup>+</sup> cells and effector NK cells which are tumor-infiltrating. It caused strong antitumor immune responses of CD8<sup>+</sup> effector cells in a tumor-antigen specific manner both in vitro and in vivo and significantly attenuated Treg cells which a control virus-infected cancer vaccine could induce. Post-treatment with this cancer vaccine after a live cancer cell injection also prominently delayed the growth of the tumor. Collectively, we demonstrate a vaccine platform consisting of BacMam virus-infected B16F10 or CT26 cancer cells that secrete IL-15:IL-15Rα. This study is the first demonstration of a functionally competent soluble IL-15:IL-15Rα complex-related cancer vaccine using a baculovirus system and advocates that the BacMam system can be used as a secure and rapid method of producing a protective and therapeutic cancer vaccine. |
topic |
interleukin 15 interleukin 15Rα B16F10 melanoma CT-26 cytokine anticancer |
url |
https://www.mdpi.com/2072-6694/13/16/4039 |
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