Direct Clinical Evidence Recommending the Use of Proteinase K or Dithiothreitol to Pretreat Sputum for Detection of SARS-CoV-2

One of the primary tools for diagnosing COVID-19 is the nucleic acid-based real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test performed on respiratory specimens. The detection rate of SARS-CoV-2 in lower respiratory specimens (such as sputum) is higher than that for upper respir...

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Bibliographic Details
Main Authors: Jing Peng, Yanjun Lu, Juan Song, Bruce A. Vallance, Kevan Jacobson, Hong Bing Yu, Ziyong Sun
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Medicine
Subjects:
DTT
Online Access:https://www.frontiersin.org/article/10.3389/fmed.2020.549860/full