An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA

An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA

This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV te...

Full description

Bibliographic Details
Main Authors: Ana Paula de Torres Santos, José Eduardo Levi, Marcilio Figueiredo Lemos, Samira Julien Calux, Isabel Takano Oba, Regina Célia Moreira
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2016-02-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
HBV viral load
Cobas Amplicor HBV monitor
Cobas TaqMan HBV
HBVDNA
in-house rtPCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000200134&lng=en&tlng=en

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000200134&lng=en&tlng=en

Similar Items