Evaluation of DNA Extraction Methods on Individual Helminth Egg and Larval Stages for Whole-Genome Sequencing

Evaluation of DNA Extraction Methods on Individual Helminth Egg and Larval Stages for Whole-Genome Sequencing

Whole-genome sequencing is being rapidly applied to the study of helminth genomes, including de novo genome assembly, population genetics, and diagnostic applications. Although late-stage juvenile and adult parasites typically produce sufficient DNA for molecular analyses, these parasitic stages are...

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Bibliographic Details
Main Authors: Stephen R. Doyle, Geetha Sankaranarayanan, Fiona Allan, Duncan Berger, Pablo D. Jimenez Castro, James Bryant Collins, Thomas Crellen, María A. Duque-Correa, Peter Ellis, Tegegn G. Jaleta, Roz Laing, Kirsty Maitland, Catherine McCarthy, Tchonfienet Moundai, Ben Softley, Elizabeth Thiele, Philippe Tchindebet Ouakou, John Vianney Tushabe, Joanne P. Webster, Adam J. Weiss, James Lok, Eileen Devaney, Ray M. Kaplan, James A. Cotton, Matthew Berriman, Nancy Holroyd
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-09-01
Series:Frontiers in Genetics
Subjects:
helminths
genomics
whole-genome sequencing
DNA extraction
low input
diagnostics
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00826/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2019.00826/full

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