Evaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia

The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram-positive bacteria bel...

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Bibliographic Details
Main Authors: Demetrio L Valle Jr, Jeannie I Andrade, Esperanza C Cabrera, Windell L Rivera
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2010-03-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000200001