MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

Cas9 fused to DNA damage repair proteins can improve rates of gene knock-in but the chimeric protein is often large. Here the authors fuse Cas9 to a minimal Brex27 motif from BRCA2 consisting of thirty-six amino acids to enhance the efficacy and safety of gene editing.

Bibliographic Details
Main Authors: Linyuan Ma, Jinxue Ruan, Jun Song, Luan Wen, Dongshan Yang, Jiangyang Zhao, Xiaofeng Xia, Y. Eugene Chen, Jifeng Zhang, Jie Xu
Format: Article
Language:English
Published: Nature Publishing Group 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19842-2

Internet

https://doi.org/10.1038/s41467-020-19842-2

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