SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells
Background. Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue. However, whether amifostine could protect neuronal cells against oxidative injury has not been reported. The purpose of this stu...
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Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2016/4202437 |
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doaj-58e4eeaa9ba64f1eb5e63151fee371c02020-11-24T22:25:33ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942016-01-01201610.1155/2016/42024374202437SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 CellsJi Jia0Lei Zhang1Xiaolei Shi2Mingchun Wu3Xiang Zhou4Xiaonan Liu5Tingting Huo6Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Anesthesiology, Xi’an No. 4 Hospital, Xi’an 710032, ChinaDepartment of General Surgery, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, ChinaDepartment of Anesthesiology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, ChinaDepartment of Anesthesiology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, ChinaDepartment of Gastrointestinal Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, ChinaBackground. Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue. However, whether amifostine could protect neuronal cells against oxidative injury has not been reported. The purpose of this study is to explore the protection of amifostine in PC12 cells. Methods. PC12 cells exposed to glutamate were used to mimic neuronal oxidative injury. SOD assay kit was taken to evaluate intracellular Cu/Zn SOD (SOD1) and SOD2 activities; western blot analysis and immunofluorescence staining were performed to investigate SOD2 protein expression; MTT, lactate dehydrogenase (LDH), release and cell morphology were used to evaluate cell injury degree, and apoptotic rate and cleaved caspase-3 expression were taken to assess apoptosis; mitochondrial superoxide production, intracellular reactive oxygen species (ROS), and glutathione (GSH) and catalase (CAT) levels were evaluated by reagent kits. Results. Amifostine increased SOD2 activity and expression, decreased cell injury and apoptosis, reduced mitochondrial superoxide production and intracellular ROS generation, and restored intracellular GSH and CAT levels in PC12 cells exposed to glutamate. SOD2-siRNA, however, significantly reversed the amifostine-induced cytoprotective and antioxidative actions. Conclusion. SOD2 mediates amifostine-induced protection in PC12 cells exposed to glutamate.http://dx.doi.org/10.1155/2016/4202437 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ji Jia Lei Zhang Xiaolei Shi Mingchun Wu Xiang Zhou Xiaonan Liu Tingting Huo |
spellingShingle |
Ji Jia Lei Zhang Xiaolei Shi Mingchun Wu Xiang Zhou Xiaonan Liu Tingting Huo SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells Oxidative Medicine and Cellular Longevity |
author_facet |
Ji Jia Lei Zhang Xiaolei Shi Mingchun Wu Xiang Zhou Xiaonan Liu Tingting Huo |
author_sort |
Ji Jia |
title |
SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells |
title_short |
SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells |
title_full |
SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells |
title_fullStr |
SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells |
title_full_unstemmed |
SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells |
title_sort |
sod2 mediates amifostine-induced protection against glutamate in pc12 cells |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2016-01-01 |
description |
Background. Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue. However, whether amifostine could protect neuronal cells against oxidative injury has not been reported. The purpose of this study is to explore the protection of amifostine in PC12 cells. Methods. PC12 cells exposed to glutamate were used to mimic neuronal oxidative injury. SOD assay kit was taken to evaluate intracellular Cu/Zn SOD (SOD1) and SOD2 activities; western blot analysis and immunofluorescence staining were performed to investigate SOD2 protein expression; MTT, lactate dehydrogenase (LDH), release and cell morphology were used to evaluate cell injury degree, and apoptotic rate and cleaved caspase-3 expression were taken to assess apoptosis; mitochondrial superoxide production, intracellular reactive oxygen species (ROS), and glutathione (GSH) and catalase (CAT) levels were evaluated by reagent kits. Results. Amifostine increased SOD2 activity and expression, decreased cell injury and apoptosis, reduced mitochondrial superoxide production and intracellular ROS generation, and restored intracellular GSH and CAT levels in PC12 cells exposed to glutamate. SOD2-siRNA, however, significantly reversed the amifostine-induced cytoprotective and antioxidative actions. Conclusion. SOD2 mediates amifostine-induced protection in PC12 cells exposed to glutamate. |
url |
http://dx.doi.org/10.1155/2016/4202437 |
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