Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
CRISPR-Cas9 has been widely adopted for genetically manipulating rodents for scientific research. Here the authors transduce mouse embryos with CRISPR-Cas9 components using rAAVs in explant culture or in vivo to produce gene-edited animals.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-02706-7 |