Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

CRISPR-Cas9 has been widely adopted for genetically manipulating rodents for scientific research. Here the authors transduce mouse embryos with CRISPR-Cas9 components using rAAVs in explant culture or in vivo to produce gene-edited animals.

Bibliographic Details
Main Authors: Yeonsoo Yoon, Dan Wang, Phillip W. L. Tai, Joy Riley, Guangping Gao, Jaime A. Rivera-Pérez
Format: Article
Language:English
Published: Nature Publishing Group 2018-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-02706-7
id doaj-591a750750ef423ca69ffb9deafcbf61
record_format Article
spelling doaj-591a750750ef423ca69ffb9deafcbf612021-05-11T09:59:19ZengNature Publishing GroupNature Communications2041-17232018-01-019111210.1038/s41467-017-02706-7Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated virusesYeonsoo Yoon0Dan Wang1Phillip W. L. Tai2Joy Riley3Guangping Gao4Jaime A. Rivera-Pérez5Department of Pediatrics, Division of Genes and Development, University of Massachusetts Medical SchoolDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical SchoolDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical SchoolDepartment of Pediatrics, Division of Genes and Development, University of Massachusetts Medical SchoolDepartment of Microbiology and Physiological Systems, University of Massachusetts Medical SchoolDepartment of Pediatrics, Division of Genes and Development, University of Massachusetts Medical SchoolCRISPR-Cas9 has been widely adopted for genetically manipulating rodents for scientific research. Here the authors transduce mouse embryos with CRISPR-Cas9 components using rAAVs in explant culture or in vivo to produce gene-edited animals.https://doi.org/10.1038/s41467-017-02706-7
collection DOAJ
language English
format Article
sources DOAJ
author Yeonsoo Yoon
Dan Wang
Phillip W. L. Tai
Joy Riley
Guangping Gao
Jaime A. Rivera-Pérez
spellingShingle Yeonsoo Yoon
Dan Wang
Phillip W. L. Tai
Joy Riley
Guangping Gao
Jaime A. Rivera-Pérez
Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
Nature Communications
author_facet Yeonsoo Yoon
Dan Wang
Phillip W. L. Tai
Joy Riley
Guangping Gao
Jaime A. Rivera-Pérez
author_sort Yeonsoo Yoon
title Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
title_short Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
title_full Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
title_fullStr Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
title_full_unstemmed Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
title_sort streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-01-01
description CRISPR-Cas9 has been widely adopted for genetically manipulating rodents for scientific research. Here the authors transduce mouse embryos with CRISPR-Cas9 components using rAAVs in explant culture or in vivo to produce gene-edited animals.
url https://doi.org/10.1038/s41467-017-02706-7
work_keys_str_mv AT yeonsooyoon streamlinedexvivoandinvivogenomeeditinginmouseembryosusingrecombinantadenoassociatedviruses
AT danwang streamlinedexvivoandinvivogenomeeditinginmouseembryosusingrecombinantadenoassociatedviruses
AT phillipwltai streamlinedexvivoandinvivogenomeeditinginmouseembryosusingrecombinantadenoassociatedviruses
AT joyriley streamlinedexvivoandinvivogenomeeditinginmouseembryosusingrecombinantadenoassociatedviruses
AT guangpinggao streamlinedexvivoandinvivogenomeeditinginmouseembryosusingrecombinantadenoassociatedviruses
AT jaimeariveraperez streamlinedexvivoandinvivogenomeeditinginmouseembryosusingrecombinantadenoassociatedviruses
_version_ 1721448828602679296

Similar Items