Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo

Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo

Abstract Objective The mammalian Notch ligand DLL1 has essential functions during development. To visualise DLL1 in tissues, for sorting and enrichment of DLL1-expressing cells, and to efficiently purify DLL1 protein complexes we tagged DLL1 in mice with AcGFPHA or Strep/FLAG. Results We generated c...

Full description

Bibliographic Details
Main Authors: Karin Schuster-Gossler, Karsten Boldt, Dorothee Bornhorst, Patricia Delany-Heiken, Marius Ueffing, Achim Gossler
Format: Article
Language:English
Published: BMC 2021-09-01
Series:BMC Research Notes
Subjects:
Mouse DLL1
DLL1 C-terminal tagging
AcGFP
StrepFLAG
DLL1 hypomorph
Online Access:https://doi.org/10.1186/s13104-021-05785-4

Internet

https://doi.org/10.1186/s13104-021-05785-4

Similar Items