The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases

The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases

A non-bifurcating NADH-dependent, dimeric [FeFe]-hydrogenase (HydAB) from Syntrophus aciditrophicus was heterologously produced in Escherichia coli, purified and characterized. Purified recombinant HydAB catalyzed NAD+ reduction coupled to hydrogen oxidation and produced hydrogen from NADH without t...

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Bibliographic Details
Main Authors: Nathaniel A. Losey, Saroj Poudel, Eric S. Boyd, Michael J. McInerney
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Microbiology
Subjects:
[FeFe]-hydrogenase
syntrophy
interspecies electron transfer
NADH-dependent hydrogen production
NADH:quinone oxidoreductase
anaerobe physiology
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01109/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.01109/full

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