Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue

Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue

To investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (¹O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV, which cleav...

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Bibliographic Details
Main Authors: Carolina Maria Berra, Carlos Frederico Martins Menck, Glaucia Regina Martinez, Carla Santos de Oliveira, Maurício da Silva Baptista, Paolo Di Mascio
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2010-01-01
Series:Química Nova
Subjects:
oxidative stress
DNA lesions
singlet molecular oxygen
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200009

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200009

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