A Novel Method of Isolating Myofibrils From Primary Cardiomyocyte Culture Suitable for Myofibril Mechanical Study

A Novel Method of Isolating Myofibrils From Primary Cardiomyocyte Culture Suitable for Myofibril Mechanical Study

Myofibril based mechanical studies allow evaluation of sarcomeric protein function. We describe a novel method of obtaining myofibrils from primary cardiomyocyte culture. Adult rat ventricular myocytes (ARVMs) were obtained by enzymatic digestion and maintained in serum free condition. ARVMs were ho...

Full description

Bibliographic Details
Main Authors: Kathleen C. Woulfe, Claudia Ferrara, Jose Manuel Pioner, Jennifer H. Mahaffey, Raffaele Coppini, Beatrice Scellini, Cecilia Ferrantini, Nicoletta Piroddi, Chiari Tesi, Corrado Poggesi, Mark Jeong
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Cardiovascular Medicine
Subjects:
myofibril
cell culture
mechanics
sarcomere
signaling
Online Access:https://www.frontiersin.org/article/10.3389/fcvm.2019.00012/full

Internet

https://www.frontiersin.org/article/10.3389/fcvm.2019.00012/full

Similar Items