Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation

Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as a gene delivery vehicle for basic research. As AAV continues to become the vector of choice, it is increasingly important for new researchers to have access to a simplified production and purificatio...

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Bibliographic Details
Main Authors: Sean M. Crosson, Peter Dib, J. Kennon Smith, Sergei Zolotukhin
Format: Article
Language:English
Published: Elsevier 2018-09-01
Series:Molecular Therapy: Methods & Clinical Development
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050118300470