Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about...

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Bibliographic Details
Main Authors: Catherine A. Hogan, ChunHong Huang, Malaya K. Sahoo, Hannah Wang, Becky Jiang, Mamdouh Sibai, Marisa Holubar, Roshni Mathew, James Zehnder, Benjamin A. Pinsky
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2021-02-01
Series:Emerging Infectious Diseases
Subjects:
SARS-CoV-2
RNA
infection control
testing
COVID-19
respiratory infections
Online Access:https://wwwnc.cdc.gov/eid/article/27/2/20-4168_article

Internet

https://wwwnc.cdc.gov/eid/article/27/2/20-4168_article

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