Novel Strategies to Optimize the Amplification of Single-Stranded DNA

The generation of single stranded DNA plays a key role in in vitro selection of DNA aptamers and in other molecular techniques such as DNA sequencing and microarrays. Here we describe three novel methodologies for ssDNA production and amplification. Furthermore, we describe some previously unnoticed...

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Bibliographic Details
Main Authors: Atef Nehdi, Nosaibah Samman, Vanessa Aguilar-Sánchez, Azer Farah, Emre Yurdusev, Mohamed Boudjelal, Jonathan Perreault
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00401/full