Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain

Here, using a humanized phage display library with recombinant SARS- CoV-2 receptor binding domain (RBD) proteins, the authors identify a number of single domain antibodies (sdAbs) that neutralize SARS-CoV-2 in vitro by inhibiting the interaction of the RDB with the host entry receptor ACE2.

Bibliographic Details
Main Authors:	Xiaojing Chi, Xiuying Liu, Conghui Wang, Xinhui Zhang, Xiang Li, Jianhua Hou, Lili Ren, Qi Jin, Jianwei Wang, Wei Yang
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-09-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-18387-8

id	doaj-5a2adb117bc54fe7a3edefdf76d0df98
record_format	Article
spelling	doaj-5a2adb117bc54fe7a3edefdf76d0df982021-09-12T11:47:26ZengNature Publishing GroupNature Communications2041-17232020-09-011111710.1038/s41467-020-18387-8Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domainXiaojing Chi0Xiuying Liu1Conghui Wang2Xinhui Zhang3Xiang Li4Jianhua Hou5Lili Ren6Qi Jin7Jianwei Wang8Wei Yang9NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing Kawin Technology Co., Ltd.Beijing Kawin Technology Co., Ltd.NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeHere, using a humanized phage display library with recombinant SARS- CoV-2 receptor binding domain (RBD) proteins, the authors identify a number of single domain antibodies (sdAbs) that neutralize SARS-CoV-2 in vitro by inhibiting the interaction of the RDB with the host entry receptor ACE2.https://doi.org/10.1038/s41467-020-18387-8
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xiaojing Chi Xiuying Liu Conghui Wang Xinhui Zhang Xiang Li Jianhua Hou Lili Ren Qi Jin Jianwei Wang Wei Yang
spellingShingle	Xiaojing Chi Xiuying Liu Conghui Wang Xinhui Zhang Xiang Li Jianhua Hou Lili Ren Qi Jin Jianwei Wang Wei Yang Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain Nature Communications
author_facet	Xiaojing Chi Xiuying Liu Conghui Wang Xinhui Zhang Xiang Li Jianhua Hou Lili Ren Qi Jin Jianwei Wang Wei Yang
author_sort	Xiaojing Chi
title	Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain
title_short	Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain
title_full	Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain
title_fullStr	Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain
title_full_unstemmed	Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain
title_sort	humanized single domain antibodies neutralize sars-cov-2 by targeting the spike receptor binding domain
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-09-01
description	Here, using a humanized phage display library with recombinant SARS- CoV-2 receptor binding domain (RBD) proteins, the authors identify a number of single domain antibodies (sdAbs) that neutralize SARS-CoV-2 in vitro by inhibiting the interaction of the RDB with the host entry receptor ACE2.
url	https://doi.org/10.1038/s41467-020-18387-8
work_keys_str_mv	AT xiaojingchi humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT xiuyingliu humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT conghuiwang humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT xinhuizhang humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT xiangli humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT jianhuahou humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT liliren humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT qijin humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT jianweiwang humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain AT weiyang humanizedsingledomainantibodiesneutralizesarscov2bytargetingthespikereceptorbindingdomain
_version_	1717755406629994496

Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain

Similar Items