Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces
Improving the long-term performance of neural electrode interfaces requires overcoming severe biological reactions such as neuronal cell death, glial cell activation, and vascular damage in the presence of implanted intracortical devices. Past studies traditionally observe neurons, microglia, astroc...
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Frontiers Media S.A.
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2019.00493/full |
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doaj-5a67e30375fe454faba01adbb286b46d2020-11-25T01:34:00ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2019-05-011310.3389/fnins.2019.00493448562Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural InterfacesSteven M. Wellman0Steven M. Wellman1Lehong Li2Yalikun Yaxiaer3Ingrid McNamara4Takashi D. Y. Kozai5Takashi D. Y. Kozai6Takashi D. Y. Kozai7Takashi D. Y. Kozai8Takashi D. Y. Kozai9Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United StatesCenter for the Neural Basis of Cognition, Pittsburgh, PA, United StatesDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United StatesEberly College of Science, Pennsylvania State University, University Park, PA, United StatesDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United StatesDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United StatesCenter for the Neural Basis of Cognition, Pittsburgh, PA, United StatesCenter for Neuroscience, University of Pittsburgh, Pittsburgh, PA, United StatesMcGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United StatesNeuroTech Center, University of Pittsburgh Brain Institute, Pittsburgh, PA, United StatesImproving the long-term performance of neural electrode interfaces requires overcoming severe biological reactions such as neuronal cell death, glial cell activation, and vascular damage in the presence of implanted intracortical devices. Past studies traditionally observe neurons, microglia, astrocytes, and blood-brain barrier (BBB) disruption around inserted microelectrode arrays. However, analysis of these factors alone yields poor correlation between tissue inflammation and device performance. Additionally, these studies often overlook significant biological responses that can occur during acute implantation injury. The current study employs additional histological markers that provide novel information about neglected tissue components—oligodendrocytes and their myelin structures, oligodendrocyte precursor cells, and BBB -associated pericytes—during the foreign body response to inserted devices at 1, 3, 7, and 28 days post-insertion. Our results reveal unique temporal and spatial patterns of neuronal and oligodendrocyte cell loss, axonal and myelin reorganization, glial cell reactivity, and pericyte deficiency both acutely and chronically around implanted devices. Furthermore, probing for immunohistochemical markers that highlight mechanisms of cell death or patterns of proliferation and differentiation have provided new insight into inflammatory tissue dynamics around implanted intracortical electrode arrays.https://www.frontiersin.org/article/10.3389/fnins.2019.00493/fulloligodendrocytesNG2 gliapericytestissue-electrode interfaceneurodegenerationgliosis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Steven M. Wellman Steven M. Wellman Lehong Li Yalikun Yaxiaer Ingrid McNamara Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai |
| spellingShingle |
Steven M. Wellman Steven M. Wellman Lehong Li Yalikun Yaxiaer Ingrid McNamara Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces Frontiers in Neuroscience oligodendrocytes NG2 glia pericytes tissue-electrode interface neurodegeneration gliosis |
| author_facet |
Steven M. Wellman Steven M. Wellman Lehong Li Yalikun Yaxiaer Ingrid McNamara Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai Takashi D. Y. Kozai |
| author_sort |
Steven M. Wellman |
| title |
Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces |
| title_short |
Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces |
| title_full |
Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces |
| title_fullStr |
Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces |
| title_full_unstemmed |
Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces |
| title_sort |
revealing spatial and temporal patterns of cell death, glial proliferation, and blood-brain barrier dysfunction around implanted intracortical neural interfaces |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Neuroscience |
| issn |
1662-453X |
| publishDate |
2019-05-01 |
| description |
Improving the long-term performance of neural electrode interfaces requires overcoming severe biological reactions such as neuronal cell death, glial cell activation, and vascular damage in the presence of implanted intracortical devices. Past studies traditionally observe neurons, microglia, astrocytes, and blood-brain barrier (BBB) disruption around inserted microelectrode arrays. However, analysis of these factors alone yields poor correlation between tissue inflammation and device performance. Additionally, these studies often overlook significant biological responses that can occur during acute implantation injury. The current study employs additional histological markers that provide novel information about neglected tissue components—oligodendrocytes and their myelin structures, oligodendrocyte precursor cells, and BBB -associated pericytes—during the foreign body response to inserted devices at 1, 3, 7, and 28 days post-insertion. Our results reveal unique temporal and spatial patterns of neuronal and oligodendrocyte cell loss, axonal and myelin reorganization, glial cell reactivity, and pericyte deficiency both acutely and chronically around implanted devices. Furthermore, probing for immunohistochemical markers that highlight mechanisms of cell death or patterns of proliferation and differentiation have provided new insight into inflammatory tissue dynamics around implanted intracortical electrode arrays. |
| topic |
oligodendrocytes NG2 glia pericytes tissue-electrode interface neurodegeneration gliosis |
| url |
https://www.frontiersin.org/article/10.3389/fnins.2019.00493/full |
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