A Robust and Efficient Production and Purification Procedure of Recombinant Alzheimers Disease Methionine-Modified Amyloid-β Peptides.

A Robust and Efficient Production and Purification Procedure of Recombinant Alzheimers Disease Methionine-Modified Amyloid-β Peptides.

An improved production and purification method for Alzheimer's disease related methionine-modified amyloid-β 1-40 and 1-42 peptides is proposed, taking advantage of the formation of inclusion body in Escherichia coli. A Thioflavin-S assay was set-up to evaluate inclusion body formation during g...

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Bibliographic Details
Main Authors: Marie Hoarau, Yannick Malbert, Romain Irague, Christelle Hureau, Peter Faller, Emmanuel Gras, Isabelle André, Magali Remaud-Siméon
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4988814?pdf=render

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http://europepmc.org/articles/PMC4988814?pdf=render

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