ExCyto PCR amplification.

ExCyto PCR cells provide a novel and cost effective means to amplify DNA transformed into competent bacterial cells. ExCyto PCR uses host E. coli with a chromosomally integrated gene encoding a thermostable DNA polymerase to accomplish robust, hot-start PCR amplification of cloned sequences without...

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Bibliographic Details
Main Authors: Vinay Dhodda, Ronald Godiska, Jeffrey D Vanwye, David Mead, Rebecca Hochstein, Lynne Sheets, Sarah Vande Zande, Chris Niebauer, Douglas L Crawford, Marjorie F Oleksiak
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-09-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2935887?pdf=render