Preparation of Adult Pig Pancreatic Cells: Comparative Study of Methods with or without Proteolytic Enzymes

Methods for the preparation of adult pig pancreatic cells using enzymatic- or auto-digestion are described, and a comparison of these methods was performed. A greater number of cells staining red with dithizone with the highest viability and insulin secretory response to glucose stimulation were obt...

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Bibliographic Details
Main Authors: Hisako Ohgawara, Akira Kobayashi, Suk-Ja Chong, Toshihiro Akaike, Yoko Hashimoto
Format: Article
Language:English
Published: SAGE Publishing 1994-07-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/096368979400300409