Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Abstract Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing effic...

Full description

Bibliographic Details
Main Authors: Yuan-Yuan Jiang, Yi-Ping Chai, Min-Hui Lu, Xiu-Li Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xue-Chen Wang, Caixia Gao, Qi-Jun Chen
Format: Article
Language:English
Published: BMC 2020-10-01
Series:Genome Biology
Subjects:
Prime editing
PE2
pegRNA
CRISPR/Cas9
Maize
ALS
Online Access:http://link.springer.com/article/10.1186/s13059-020-02170-5

Internet

http://link.springer.com/article/10.1186/s13059-020-02170-5

Similar Items